The reproducibility crisis in biomedical research prompts demand for measurement standards to enable comparability and traceability of measurement results. Many scientific advancements have been achieved to elucidate the underpinning processes of biological function, which occur at subcellular and cellular levels. However, the metrology foundations of such processes remain sparse to keep pace with response to evolving healthcare challenges, ranging from pandemics to neurodegeneration.

Of particular relevance to this is the persistent need for reference materials exhibiting nanoscale periodicities over microscopic length scales, with traceability established to the International System of Units (Système International d’Unités – the SI). These materials are required to calibrate high-resolution instruments, such as electron microscopes, and enable traceable measurements of biological samples, such as tissue biopsies.

This short introductory course will demonstrate the use of such SI-traceable materials, exemplified by a case study of bio-calibrants developed for high-resolution microscopy. These types of materials can be used in any application areas that require measurements with a nanoscale accuracy. Various nanoscale imaging techniques are explored and the basic principles of traceability are introduced. The course then focuses on the application of reference materials to validate measurement results at laboratory scales. This is supported by a selection of case studies to demonstrate how such principles are applied in both industrial and clinical settings.

Learners who successfully complete the course will receive an NPL Certificate of Completion.